Journal: Theranostics

Article Title: Quantitative MALDI imaging of aspirin metabolites in mouse models of triple-negative breast cancer

doi: 10.7150/thno.116819

Figure Lengend Snippet: Quantification of SA in liver, kidney, and SUM159 tumor tissue sections. ( A ) MALDI images of salicylic acid dried droplets (SA, [M-H]⁻, m/z 137.02 Da, 0.5-150 pmol/mm²) were spotted onto untreated tissue sections, measured by QMALDI imaging in negative ion mode, and are shown as normalized SA images of the SA-to-D 6 -SA ratio. ( B ) Calibration curves of SA were generated using QMALDI imaging in negative ion mode with D 6 -SA as deuterated internal standard and peracetic acid as additive. SA concentration plotted versus SA-to-D 6 -SA intensity ratios generated calibration curves. MS intensities were derived from the average spectra of each circled SA dried droplet spot for each SA concentration as shown in panel A. ( C ) MALDI images of D 6 -SA and normalized images of SA-to-D 6 -SA ratio were acquired and are shown from data analysis in SCiLS lab. The measured concentrations of SA in the liver, kidney, and SUM159 tumor tissues of mice injected with 300 mM aspirin were 141.9 ± 22.6 pmol/mm², 129.5 ± 7.8 pmol/mm², and 50.4 ± 3.0 pmol/mm², respectively, as shown in panels B and C. The results are presented as mean ± SD from three independent technical replicates of tissues.

Article Snippet: MALDI imaging data were processed using SCiLS Lab software (version 2024b, Bruker Daltonics).

Techniques: Imaging, Generated, Concentration Assay, Derivative Assay, Injection

Journal: Theranostics

Article Title: Quantitative MALDI imaging of aspirin metabolites in mouse models of triple-negative breast cancer

doi: 10.7150/thno.116819

Figure Lengend Snippet: QMALDI SA imaging data analysis integrated with H-E and CD31 immunofluorescence staining to quantify SA content in necrotic, viable, and vascularized tumor regions. ( A ) The H-E and optical images are from the same tissue sections as the MALDI image, while the DAPI and CD31 images are from adjacent serial tissue sections. The figure includes three biological replicates of H-E, CD31, and DAPI-stained tumor sections, paired with MALDI images showing the spatial distribution of salicylic acid (SA) ( m/z 137.02 Da, [M-H]⁻) in tissues from aspirin-treated mice. A merged image displaying the co-registration of the CD31 image with H-E-stained viable and necrotic regions in SUM159 tumors is shown in the leftmost images. ( B ) DAPI and CD31 images were co-registered with and down sampled to match the MALDI imaging data, enabling precise overlay and alignment. The integration of DAPI and CD31 immunofluorescence (IF) images with MALDI images was performed using the Weave software, allowing for a detailed spatial comparison across these different imaging modalities. The integrated image was analyzed by classifying regions into high-CD31(magenta) and low-CD31 (cyan) areas, followed by calculating the average normalized SA intensity for each region. ( C ) H-E-stained images of viable (blue) and necrotic (red) regions in SUM159 tumors were co-registered with MALDI images of SA using the SCiLS Lab software. The average normalized SA intensity was calculated for each region from these co-registered images. All presented measurements were performed by quantifying the SA intensity per pixel, where each open circle represents one pixel in the underlying violin plots. MALDI imaging was performed using a timsTOF fleX MALDI-2 instrument with norharmane matrix and peracetic acid additive at a 20 µm pixel size in negative ion mode. All results are also presented as mean ± standard deviation (SD) where the mean value for each group is indicated by yellow squares.

Article Snippet: MALDI imaging data were processed using SCiLS Lab software (version 2024b, Bruker Daltonics).

Techniques: Imaging, Immunofluorescence, Staining, Software, Comparison, Standard Deviation

Journal: Theranostics

Article Title: Quantitative MALDI imaging of aspirin metabolites in mouse models of triple-negative breast cancer

doi: 10.7150/thno.116819

Figure Lengend Snippet: QMALDI SA imaging data analysis integrated with H-E staining to quantify SA content in anatomical kidney regions. ( A ) The H-E and optical images are from the same tissue sections as the MALDI image. The figure includes three biological replicates of H-E-stained kidney sections, paired with MALDI images showing the spatial distribution of salicylic acid (SA) ( m/z 137.02 Da, [M-H]⁻) in tissues from aspirin-treated mice. ( B ) H-E-stained images of medulla (yellow) and cortex (blue) regions in kidneys were co-registered with MALDI images of SA using SCiLS Lab software. The average normalized SA intensity was calculated for each region from these co-registered images. All presented measurements were performed by quantifying the SA intensity per pixel, where each open circle represents one pixel in the underlying violin plots. MALDI imaging was performed using a timsTOF fleX MALDI-2 instrument with norharmane matrix and peracetic acid additive at a 20 µm pixel size in negative ion mode. All results are also presented as mean ± standard deviation (SD) where the mean value for each group is indicated by yellow squares.

Article Snippet: MALDI imaging data were processed using SCiLS Lab software (version 2024b, Bruker Daltonics).

Techniques: Imaging, Staining, Software, Standard Deviation

Journal: Scientific Reports

Article Title: Emergence of highly virulent multidrug and extensively drug resistant Escherichia coli and Klebsiella pneumoniae in buffalo subclinical mastitis cases

doi: 10.1038/s41598-025-95914-x

Figure Lengend Snippet: The overall phenotype-genotype correlation of E. coli ( a ) and K. pneumoniae ( b ) positive isolates. Biofilm production was represented as a percentage (%) using a radar map ( c ). The figures ( a , b ) were created using RStudio (R 4.3.3) with the Metan package, while the radar map was generated using Origin 2024b.

Article Snippet: The figures ( a , b ) were created using RStudio (R 4.3.3) with the Metan package, while the radar map was generated using Origin 2024b.

Techniques: Generated